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Identification of intracellular targets of small molecular weight chemical compounds using affinity chromatography
Author(s) -
Guiffant Damien,
Tribouillard Déborah,
Gug Fabienne,
Galons Hervé,
Meijer Laurent,
Blondel Marc,
Bach Stéphane
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600223
Subject(s) - intracellular , small molecule , anticipation (artificial intelligence) , chemistry , affinity chromatography , drug discovery , drug , enzyme , computational biology , selectivity , identification (biology) , biochemistry , biology , pharmacology , computer science , botany , artificial intelligence , catalysis
Efforts to characterize small molecular weight chemical inhibitors of pharmacological interest tend to identify molecules with high efficiency and selectivity, to meet the two criteria required for the clinical development of a drug: efficacy and harmlessness. Drug candidates are expected to inhibit efficiently the target they have been optimized against (for example, a particular type of protein kinase). These hits are also designed to not interfere (or as little as possible) with the activity of other cellular enzymes/proteins to reduce undesired side effects. Here we discuss the use of immobilized drugs as affinity chromatography matrices to purify and identify their bona fide intracellular targets. This method not only allows the systematic investigation of the selectivity of pharmacological compounds but also the anticipation of their putative adverse effects.