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Methods to produce marker‐free transgenic plants
Author(s) -
Darbani Behrooz,
Eimanifar Amin,
Stewart C. Neal,
Camargo William N.
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600182
Subject(s) - selectable marker , transgene , transformation (genetics) , marker gene , biology , gene , genetically modified crops , computational biology , herbicide resistance , microbiology and biotechnology , selection (genetic algorithm) , genetics , computer science , artificial intelligence
Selectable marker genes (SMGs) have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. The most used SMGs encode proteins resistant to antibiotics or herbicides and use negative selection, i.e. , by killing nontransgenic tissue. However, there are perceived risks in wide‐scale deployment of SMG‐transgenic plants, and therefore research has recently been performed to develop marker‐free systems. In this review, transformation using markers not based on antibiotic or herbicide resistance genes, as well as different systems of marker gene deletion, are discussed.