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Bio‐electrosprays: The next generation of electrified jets
Author(s) -
Jayasinghe Suwan N.,
TownsendNicholson Andrea
Publication year - 2006
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600128
Subject(s) - electrospray , nanotechnology , biochemical engineering , drop (telecommunication) , living cell , jet (fluid) , chemistry , materials science , biophysics , biological system , computer science , biology , mechanics , chromatography , physics , engineering , mass spectrometry , telecommunications
Biological electrosprays are rapidly becoming a robust means by which to engineer living organisms for applications ranging from tissue repair to developmental biology. We previously reported the ability to electrospray living organisms without compromising their viability, but found it challenging to achieve stability in the jetting of these organisms as a result of the chemical properties of the living cellular suspensions. Jet stability is required for the generation of a near‐mono distribution of droplets, which is necessary for the development of electrospray technology as a “drop and place” biotechnique. Recently, we determined the conditions needed to achieve jet stability and were able to generate droplets with a near‐mono distribution (<50 μm). In this communication, we elucidate the relationship between jet behaviour and droplet size under stable jetting conditions, with a view to further reducing the droplet size to deposit a single living cell within a droplet. We believe that this level of resolution will make electrospray jetting superior amongst the jet‐based biotechnologies presently being developed for the engineering of biological architectures comprised of living cells.