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Enhanced cutinase production with Thermobifida fusca by two‐stage pH control strategy
Author(s) -
Du Guocheng,
Zhang Shouliang,
Hua Zhaozhe,
Zhu Yang,
Chen Jian
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600122
Subject(s) - cutinase , fermentation , chemistry , yield (engineering) , carbon source , biochemistry , food science , enzyme , materials science , metallurgy
A mutant of Thermobifida fusca ATCC 27730 was used for cutinase production. Acetate was the most suitable carbon source for cell growth and cutinase production compared with others. The pH was one of the most important factors affecting cutinase yield and productivity. Batch cutinase fermentations by mutant Thermobifida fusca WSH04 at various pH values ranging from 7.0 to 7.9 were studied. Based on the effects of different pH values on the specific cell growth rate and specific cutinase formation rate, a two‐stage pH control strategy was developed, in which the pH was set at 7.3 for the first 20 h, and switched to 7.6 afterwards. By applying this two‐stage pH control strategy for cutinase fermentation, the maximal cutinase activity reached 19.8 U/mL.