Premium
A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei
Author(s) -
Ouyang Jia,
Yan Ming,
Kong Dechong,
Xu Lin
Publication year - 2006
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600103
Subject(s) - trichoderma reesei , cellulase , trichoderma , genome , gene , biology , glycoside hydrolase , biochemistry , cellulose , orfs , fungal protein , microbiology and biotechnology , saccharomyces cerevisiae , peptide sequence , botany , open reading frame
The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome‐wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose‐binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel β‐glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose‐degrading proteins that have highly conserved CBMs; and group D containing β‐glucosidase and β‐xylosidase. Group D was the largest group, in which 8 β‐glucosidases appeared to be non‐secreted proteins.