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Production of galacto‐oligosaccharides by immobilized recombinant β‐galactosidase from Aspergillus candidus
Author(s) -
Zheng Pu,
Yu Hongfeng,
Sun Zhihao,
Ni Ye,
Zhang Wei,
Fan Yunliu,
Xu Yan
Publication year - 2006
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600100
Subject(s) - lactose , dilution , yield (engineering) , chemistry , chromatography , recombinant dna , food science , biochemistry , materials science , physics , gene , metallurgy , thermodynamics
The preparation of galacto‐oligosaccharides (GOSs) was studied using the immobilized recombinant β‐galactosidase from Aspergillus candidus CGMCC3.2919. The optimal pH and temperature for the immobilized enzyme were observed at pH 6.5 and 40°C, respectively. Increasing the initial lactose concentration increased the yield of GOSs. The dilution rate was found to be a key factor during the continuous production of GOSs. The maximum productivity, 87 g/L·h was reached when 400 g/L lactose was fed at dilution rate of 0.8/h. The maximum GOS yield reached 37% at dilution rate of 0.5/h. Continuous operation was maintained for 20 days in a packed‐bed reactor without apparent decrease in GOS production. The average yield of GOSs was 32%, corresponding to the average productivity of 64 g/L·h, which implied that the immobilized recombinant β‐galactosidase has potential application for GOS production.