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Analysis of the products from enzymatic scouring of cotton
Author(s) -
Saebe Porntip,
Sangwatanaroj Usa,
Punnapayak Hunsa
Publication year - 2007
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200600080
Subject(s) - pectinase , cellulase , behenic acid , lipase , protease , hydrolysis , chemistry , stearic acid , food science , chromatography , enzyme , palmitic acid , fatty acid , biochemistry , organic chemistry
This article discusses the analysis of the hydrolysis products from one‐step scouring of cotton using pectinase and two‐step scouring of cotton using lipase then cellulase, protease then cellulase, or lipase/protease then cellulase, to improve water absorbency of cotton. UV spectrophotometric analysis indicated that the pectinase scouring process produced approximately 18‐fold higher amounts of reducing sugars and galacturonic acid than any of the two‐step scouring processes. The production rate of reducing sugars and galacturonic acid from most of the scouring processes showed a decrease with an increase in time. HPLC analysis revealed that the lipase/protease/cellulase scouring processes produced approximately 5‐fold higher amounts of 17 amino acids than the pectinase scouring process. GC analysis for 18 fatty acids (C 8 ‐C 24 ) revealed that three major fatty acids, palmitic acid, stearic acid, and behenic acid, were found on both the scoured and the unscoured fabrics. Scoured fabrics were tested for content of proteins, extractable components, waxes, and anionic components including pectins, and some differences among the fabric scoured with different enzyme combinations were found.