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Production of D ‐arabitol by a metabolic engineered strain of Bacillus subtilis
Author(s) -
Povelainen Mira,
Miasnikov Andrei N.
Publication year - 2006
Publication title -
biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.144
H-Index - 84
eISSN - 1860-7314
pISSN - 1860-6768
DOI - 10.1002/biot.200500035
Subject(s) - bacillus subtilis , biochemistry , strain (injury) , metabolic engineering , mutant , chemistry , acetoin , dehydrogenase , biology , microbiology and biotechnology , enzyme , fermentation , gene , bacteria , genetics , anatomy
A novel method for D ‐arabitol production with a metabolically engineered Bacillus subtilis strain is described. A known transketolase‐deficient and D ‐ribose‐producing mutant of B. subtilis (ATCC 31094) was further modified by disruption of its rpi ( D ‐ribose phosphate isomerase) gene to create a D ‐ribulose‐ and D ‐xylulose‐producing B. subtilis strain. Expression of the D ‐arabitol phosphate dehydrogenase gene of Enterococcus avium in the D ‐ribulose‐ and D ‐xylulose‐producing strain resulted in a strain of B. subtilis capable of converting D ‐glucose to D ‐arabitol with a high yield (38%) and little by‐product formation.