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Black tea extract suppresses transformation of aryl hydrocarbon receptor induced by dioxin
Author(s) -
Fukuda Itsuko,
Sakane Iwao,
Yabushita Yoshiyuki,
Sawamura ShinIchi,
Kanazawa Kazuki,
Ashida Hitoshi
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.552210170
Subject(s) - aryl hydrocarbon receptor , chemistry , transformation (genetics) , black tea , ethanol , cytosol , receptor , toxicity , ic50 , pharmacology , hydrocarbon , biochemistry , in vitro , food science , biology , transcription factor , organic chemistry , gene , enzyme
Abstract Dioxins cause various adverse effects through binding to an aryl hydrocarbon receptor (AhR) and transformation of the receptor. In this study, we investigated whether black tea extract suppresses AhR transformation. Dried black tea leaves were extracted with 75% ethanol, and the extract was pretreated to the rat liver cytosol fraction 10 min prior to addition of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD). Transformed AhR was detected by electrophoretic gel mobility shift assay. Black tea extract suppressed AhR transformation in a dose‐dependent manner, and the IC 50 value against 1 nM TCDD‐induced AhR transformation was 8.9 μ/ml. The result suggests that intake of black tea has a potential to suppress the AhR transformation, leading protection from dioxin toxicity.

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