Premium
Abies nephrolepis leaf phenolics prevent the inhibition of gap junction intercellular communication by hydrogen peroxide in rat liver epithelial cells
Author(s) -
Lee Sang Jun,
Lee Ki Won,
Lee Hyong Joo
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.552210168
Subject(s) - butylated hydroxytoluene , hydrogen peroxide , catechin , chemistry , epigallocatechin gallate , intracellular , antioxidant , biochemistry , tumor promotion , polyphenol , botany , carcinogenesis , biology , gene
Recent reports suggest that carcinogenicity of hydrogen peroxide (H 2 O 2 ) is implicated in inhibition of gap junction intercellular communication (GJIC), which is a cellular event associated with the tumor promotion. The present study investigated the effect of phenolics (KF) from leaves of Abies nephrolepis (Khingan fir) on inhibition of GJIC by H 2 O 2 in WB‐F344 rat liver epithelial cells. The phenolics were extracted from fresh leaves by using 80% aqueous methanol, and were analyzed mainly as catechin derivatives including epigallocatechin gallate (EGCG) and catechin itself. KF and EGCG protected the inhibition of GJIC by H 2 O 2 , whereas butylated hydroxytoluene, a commercial antioxidant, had no effect. Our results indicate that KF exhibits potential chemopreventive effects against carcinogenesis, which may be attributable to phenolics such as EGCG.