Premium
Effect of green tea catechins on oxidative DNA damage of hamster pancreas and liver induced by N‐Nitrosobis(2‐oxopropyl)amine and/or oxidized soybean oil
Author(s) -
Takabayashi Fumiyo,
Tahara Shoichi,
Kaneko Takao,
Harada Noboru
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.552210165
Subject(s) - soybean oil , deoxyguanosine , hamster , pancreas , chemistry , medicine , carcinogenesis , endocrinology , carcinogen , dna damage , oxidative stress , food science , biochemistry , dna , gene
It has been indicated that high fat diet is a risk factor of the pancreatic cancer by epidemiological studies. We examined whether the oxidized soybean oil (ox‐oil) express the synergistic effect on the formation of 8‐ox O 2 − '‐deoxyguanosine (8‐oxodG) in nuclear DNA of hamster pancreas induced by N‐Nitrosobis(2‐oxopropyl)amine (BOP) and whether the green tea catechins (GTC) suppressed it. Ox‐oil was prepared by air oxidation, and the content of lipid hydroperoxide was 6.22 mg/ml. Hamsters were administered 0.3∼ml of ox‐oil/day orally for 4 weeks before BOP treatment. GTC was given ad libitum as a 0.1% aqueous solution. Four hours after subcutaneous administration of BOP, hamsters were sacrificed, and the contents of 8‐oxodG were measured in nuclear DNA of pancreas and liver. The 8‐oxodG content in the pancreas was increased by BOP and/or ox‐oil administration. However, it was not suppressed by an intake of GTC. In the liver, though the content of 8‐oxodG was increased by ox‐oil, it tended to suppress the rise of 8‐oxodG by a GTC intake. These results suggested that the long term intake of ox‐oil might have the possibility to induce carcinogenesis in hamster pancreas and liver, and an intake of GTC might have the beneficial effect on liver.