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Regulation of interleukin‐8 secretion in human intestinal epithelial Caco‐2 cells by α‐humulene
Author(s) -
Satsu Hideo,
Matsuda Tomoko,
Toshimitsu Takayuki,
Mori Akira,
Mae Tatsumasa,
Tsukagawa Misuzu,
Kitahara Mikio,
Shimizu Makoto
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.552210127
Subject(s) - secretion , humulene , tumor necrosis factor alpha , biology , interleukin , proinflammatory cytokine , immune system , cytokine , endocrinology , medicine , microbiology and biotechnology , chemistry , immunology , inflammation , food science , essential oil
It is well known that various cytokines such as interleukin‐8 (IL‐8) and tumor necrosis factor‐α (TNF‐α are expressed and secreted from intestinal epithelial cells and that these cytokines affect the immune cells beneath the intestinal epithelial monolayers. As the secretion of these cytokines is likely to be regulated by food‐derived substances, we focused on those food substances which regulate the secretion of IL‐8 in human intestinal epithelial Caco‐2 cells. 72 food samples extracted with 40% ethanol were tested, and the extracts of peppermint and dokudami significantly increased the IL‐8 secretion. Among the compounds known to be contained in peppermint and dokudami, α‐humulene substantially increased the IL‐8 secretion.α‐Humulene had no significant effect on the secretion of such other soluble factors as TNF‐α, IL‐1β, IL‐6, or NGF, suggesting that the effect of α‐humulene was specific for IL‐8 secretion. The expression level of IL‐8 mRNA was significantly increased by treating with α‐humulene. These results suggest that the secretion of IL‐8 by α‐humulene is regulated at the transcriptional level.