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Effects of major dietary antioxidants on inflammatory markers of RAW 264.7 macrophages
Author(s) -
Jung WonJin,
Sung MiKyung
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.552210122
Subject(s) - quercetin , antioxidant , nitrite , nitric oxide synthase , chemistry , nitric oxide , pharmacology , anti inflammatory , biochemistry , oxidative stress , biology , nitrate , enzyme , organic chemistry
Oxidative stresses are involoved in the process of chronic inflammatory diseases. The objective of this study was to evaluate and compare the anti‐inflammatory activity of major dietary antioxidants. Murine RAW264.7 macrophages (4 × 10 6 cells) were incubated in DMEM containing 10% FBS supplemented with 5 to 20 μM of α‐tocopherol, β‐carotene or quercetin for 24 hrs, and then treated with LPS for 16hrs. The medium was collected to measure TNF‐α, IL‐6, PGE 2 and nitrite. Expressions of cyclooxygenase‐2(COX‐2) and inducible nitric oxide synthase(iNOS) were determined using the harvested cells. Results indicate that TNF‐α and IL‐6 accumulations were significantly reduced by 5 to 20 μM quercetin treatment, and 20 μM of α‐tocopherol treatment. Nitrite release was significantly reduced by 5 μM quercetin treatment. However, PGE 2 accumulation was not affected by any of the antioxidants used. Expressions of COX‐2 and iNOS were effectively reduced by 5 μM quercetin treatment. These findings indicate that dietary antioxidants possess significant anti‐inflammatory activities, and quercetin is the most potent antioxidant.