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Modulation of energy metabolic enzyme expression in N‐nitrosodiethylamine‐mediated hepatocarcinogenesis by Chinese herbs, Huqi San
Author(s) -
Wen Zhaoyang,
Shi Zhengming,
Feng Ping,
Xue Xiaowei,
Dong Kun,
Wang Xuejiang
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520340406
Subject(s) - 2 acetylaminofluorene , atpase , chemistry , body weight , medicine , enzyme , endocrinology , adenosine triphosphatase , immunofluorescence , biochemistry , biology , immunology , microsome , antibody
Aim : To elucidate the anticancer mechanism of Huqi San by assessing the expression of G‐6‐Pase, SDH, ATPase and AFP in N‐nitrosodiethylamine‐mediated hepatocarcinogenesis in rats. Methods : A Solt‐Farber two‐step test model of hepatocarcino genesis was established by diethylnitrosamine (DEN) and 2‐acetylaminofluorene (AAF) in rats to investigate the modifying effects of expression of 6‐glucosephosphatase (G‐6‐Pase), succinodehydrogenase (SDH), adenosine triphosphatase (ATPase) in N‐nitrosodiethylamine‐mediated hepatocarcinogenesis. Hu Qisan compounded by eight medicinal herbs was prepared in glycoprival granules with wich 0.38 g crude herbs/mL solution was prepared for administration. γ‐Glutamy‐transpeptidase (γ‐GT), G‐6‐Pase, SDH and ATPase were immunohistochemically determined. The activity of alpha‐fetoglobulin (AFP) in the livers was measured with Immunofluorescence. Results : Huqi San treated rats showed significant decrease in areas of ‐GT positive foci ( P < 0.001). On the other hand, the expression of G‐6‐Pase, SDH and ATPase has obviously altered in Huqi San treated group. The activity of AFP also significantly decreased after the treatment with Huqi San (8 g/kg body weight or 4 g/kg body weight) or total alkali of mistletoe (0.12 g/kg body weight).