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Effect of Ccapsaicin on tNOX (ENOX2) protein expression in stomach cancer cells
Author(s) -
Wang HsiMing,
Chueh Pin Ju,
Chang ShengPang,
Yang ChiLien,
Shao KuoNing
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520340305
Subject(s) - cell growth , apoptosis , transfection , cancer cell , hela , biology , cell culture , cancer research , rna interference , cell , cancer , microbiology and biotechnology , pharmacology , rna , biochemistry , gene , genetics
Tumor‐associated NADHoxidase (tNOX, also known as ENOX2) is a growth‐related protein expressed in transformed cells. Previous reports have revealed that the inhibition of tNOX activity by the anti‐cancer drug, capsaicin, correlates with a reduction in growth of cancer cells, indicating a close relationship between tNOX activity and cell growth. Moreover, the study of depleted tNOX expression by RNA interference in HeLa cells suggests that it may be associated with the ability of tumor cells to acquire an aggressive phenotype, particularly in relation to cell proliferation. A key role for tNOX in regulating cell growth is further supported by the observation that the growth rate of MEF cells from tNOX‐overexpressing transgenic mice is approximately two‐fold greater than that of wild‐type cells. The purpose of this study was to investigate the anti‐proliferative effect of capsaicin on tNOX expression level in stomach cancer cells. We showed that capsaicin induced cytotoxicity in SCM cells concomitantly with apoptosis, PARP cleavage, and down‐regulation of tNOX protein.