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New techniques to detect oxidative stress markers: Mass spectrometry‐based methods to detect isoprostanes as the gold standard for oxidative stress in vivo
Author(s) -
Yin Huiyong
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520340203
Subject(s) - oxidative stress , isoprostanes , lipid peroxidation , in vivo , chemistry , isoprostane , oxidative phosphorylation , lipidomics , biochemistry , biomarker , biology , microbiology and biotechnology
Free radical‐induced lipid oxidation under oxidative stress has been implicated in a number of human diseases. Isoprostanes (IsoPs), isomers of prostaglandins, are one of the major classes of oxidation products derived from this oxidation process. Measurement of the levels of IsoPs by Mass Spectrometry‐based methods has become the “gold standard” biomarker of oxidative stress in vivo . Significant advances have been made in understanding this important pathway of lipid peroxidation since the discovery of IsoP formation in vivo 18 years ago. Studies from our laboratory and others are discussed that have provided insights into the mechanism of formation. Furthermore, new independent studies have demonstrated that IsoPs are the most reliable available marker of lipid peroxidation in vivo , and recent work examining IsoP formation has provided valuable information about the pathogenesis of numerous human diseases. Thus, the complexity of the IsoP pathway has expanded, providing novel insights into mechanisms of lipid peroxidation in vivo and allowing investigators to explore the role of oxidative stress in human disease.