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Bilberry extract reduces UVA‐induced oxidative stress in HaCaT keratinocytes: A pilot study
Author(s) -
Svobodová Alena,
Rambousková Jana,
Walterová Daniela,
Vostalová Jitka
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520330402
Subject(s) - hacat , vaccinium myrtillus , bilberry , reactive oxygen species , antioxidant , chemistry , oxidative stress , polyphenol , pharmacology , lipid peroxidation , biochemistry , food science , botany , biology , in vitro
Exposure to UVA radiation is known to cause many adverse biological effects by inducing the stricken cells to produce reactive oxygen species (ROS). In recent years the use of botanicals has received considerable interest in the skin protection. Bilberry ( Vaccinium myrtillus L.) fruit contains several polyphenols with strong antioxidant and anti‐inflammatory properties. In this study we evaluated potential UVA preventive effect of V. myrtillus fruit extract (VME; anthocyanins, 25% w/w) in HaCaT keratinocytes. Pre‐treatment (1 h) or post‐treatment (4 h) of HaCaT with VME resulted in attenuation of UVA‐caused damage. Application of the extract significantly reduced UVA‐stimulated ROS formation in keratinocytes. VME also prevented/reduced UVA‐caused peroxidation of membrane lipids and depletion of intracellular GSH. The observed cytoprotective effect may be linked to the antioxidant activity of the plant constituents, namely anthocyanins.