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Effect of quercetin on oxidative nuclear and mitochondrial DNA damage
Author(s) -
Potenza Lucia,
Calcabrini Cinzia,
De Bellis Roberta,
Mancini Umberto,
Cucchiarini Luigi,
Dachà Marina
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520330104
Subject(s) - oxidative damage , mitochondrial dna , nuclear dna , quercetin , chemistry , oxidative phosphorylation , dna damage , oxidative stress , mitochondrion , dna , biochemistry , antioxidant , gene
Quercetin is a well‐investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively‐injured DNA. Cell‐free and cell studies were performed. Cell‐free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability.