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Positive inotropic effect of coenzyme Q 10 , omega‐3 fatty acids and propionyl‐L‐carnitine on papillary muscle force‐frequency responses of BIO TO‐2 cardiomyopathic Syrian hamsters
Author(s) -
Vargiu Romina,
Littarru Gian Paolo,
Faa Gavino,
Mancinelli Rino
Publication year - 2008
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520320116
Subject(s) - inotrope , carnitine , medicine , endocrinology , omega , carnitine o palmitoyltransferase , chemistry , coenzyme a , beta oxidation , metabolism , biochemistry , physics , enzyme , quantum mechanics , reductase
The inability of heart muscle to generate ventricular pressure to adequately propel blood through the cardiovascular system is a primary defect associated with congestive heart failure (CHF). Force‐frequency relationship (FFR) is one of the main cardiac defects associated with congestive heart failure. Thus FFR is a convenient methodological tool for evaluating the severity of muscle contractile dysfunction and the effectiveness of therapeutic agents. Papillary muscle isolated from BIO TO‐2 cardiomyopathic Syrian hamsters (CMSHs), show a depressed FFR and represents an animal model of human idiopathic dilated cardiomyopathy. In the present study we investigated the effect of CoQ 10 , omega‐3 fatty acids, propionyl‐L‐carnitine (PLC) and a combination of these 3 agents (formulation HS12607) on FFR in 8 month old BIO TO‐2 CMSHs. Papillary muscles isolated from the anesthetized animals were placed in an incubation bath and attached to an isometric force transducer. A digital computer with an analog/digital interface allowed control of both muscle developed force and electrical stimulus parameters. Force‐frequency response was evaluated, at L max , with increasing frequencies: 0.06, 0.12, 0.25, 0.5, 1, 2 and 4 Hz. HS12607‐treatment produced a positive inotropic effect resulting in a significant enhancement ( p < 0.05) of the peak force at the highest frequencies (1–4 Hz). In the range of frequency of 1–4 Hz also CoQ 10 and omega‐3 significantly( p < 0.05) attenuated the fractional decline in developed force. The significant improvement ( p < 0.05) of the timing parameter peak rate of tension rise (+T') and peak rate of tension fall (−T') indicating a faster rate of muscle contraction and relaxation respectively, found in CoQ 10 , omega‐3 and PLC‐treated CMSHs, may be due to the positive effects of these substances on sarcoplasmic reticulum functions. These findings suggest that naturally occurring CoQ 10 , omega‐3 and PLC, particularly when administered together in a coformulation, might be a valid adjuvant to conventional therapy in dilated cardiomyopathy especially when considering that they are natural substances, devoid of side effects.