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Influence of Coenzyme Q 10 on release of pro‐inflammatory chemokines in the human monocytic cell line THP‐1
Author(s) -
Schmelzer Constance,
Lorenz Gerti,
Rimbach Gerald,
Döring Frank
Publication year - 2007
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520310308
Subject(s) - thp1 cell line , chemokine , secretion , monocyte , cell culture , macrophage inflammatory protein , chemistry , in vitro , inflammation , microbiology and biotechnology , biology , biochemistry , immunology , genetics
Coenzyme Q 10 (CoQ 10 ) is an obligatory element in the mitochondrial electron transport system and functions as a potent antioxidant of lipid membranes. In‐vivo and in‐vitro studies indicate an involvement of CoQ 10 in inflammatory pathways. Here we studied in the human monocytic cell‐line THP‐1 the influence of CoQ 10 on LPS‐induced secretion of the pro‐inflammatory chemokines Macrophage inflammatory protein‐1 alpha (MIP‐1α), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemoattractant protein‐1 (MCP‐1). In comparison to unstimulated cells, LPS leads to 22‐, 3‐ and 4.5‐fold higher levels of MIP‐1α, RANTES and MCP‐1 in the cell culture medium, respectively. Pre‐incubation of cells with 10 μM CoQ 10 resulted in a significant decrease of LPS‐induced MIP‐1α and RANTES secretion to 55.04% ( p = 0.02) and 76.84% ( p = 0.04), respectively. In conclusion, CoQ 10 reduces the LPS‐induced secretion levels of the pro‐inflammatory chemokines MIP‐1α and RANTES in the human monocytic cell line THP‐1. These data suggest that CoQ 10 possesses anti‐inflammatory properties.