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Effects of antioxidant stobadine on protein carbonylation, advanced oxidation protein products and reductive capacity of liver in streptozotocin‐diabetic rats: Role of oxidative/nitrosative stress
Author(s) -
Cumaoǧlu Ahmet,
Cevik ÇEmal,
Rackova Lucia,
Ari Nuray,
Karasu ÇImen
Publication year - 2007
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520300304
Subject(s) - oxidative stress , protein carbonylation , streptozotocin , diabetes mellitus , antioxidant , thiol , medicine , chemistry , endocrinology , nitrotyrosine , oxidative phosphorylation , pharmacology , lipid peroxidation , biochemistry , nitric oxide , nitric oxide synthase
Background : Increased oxidative/nitrosative stress is important in the pathogenesis of diabetic complications, and the protective effects of antioxidants are a topic of intense research. The purpose of this study was to investigate whether a pyridoindole antioxidant stobadine (STB) have a protective effect on tissue oxidative protein damage represented by the parameters such as protein carbonylation (PC), protein thiol (P‐SH), total thiol (T‐SH) and non‐protein thiol (Np‐SH), nitrotyrosine (3‐NT), and advanced oxidation protein products (AOPP) in streptozotocin‐diabetic rats. Methods : Diabetes was induced in male Wistar rats by intraperitonal injection of streptozotocin (55 mg/kg). Some of the non‐diabetic (control) and diabetic rats treated with STB (24.7 mg/kg/day) during 16 weeks, and the effects on blood glucose, PC, AOPP, 3‐NT, P‐SH, T‐SH and Np‐SH were studied. Biomarkers were assayed by enzyme‐linked immunosorbent assay (ELISA) or by colorimetric methods. Results : Administration of stobadine to diabetic animals lowered elevated blood glucose levels by ∼16% relative to untreated diabetic rats. Although stobadine decreased blood glucose, poor glycemic control was maintained in stobadine treated diabetic rats during the treatment period. Biochemical analyses of liver proteins showed significant diminution of sulfhydryl groups, P‐SH, T‐SH, Np‐SH, and elevation of carbonyl groups in diabetic animals in comparison to healthy controls. As a biomarker of nitrosative stress, 3‐NT levels did not significantly change by diabetes induction or by stobadine treatment when compared to control animals. However, the treatment with stobadine resulted in a significant decrease in PC, AOPP levels and normalized P‐SH, T‐SH, Np‐SH groups in liver of diabetic animals.