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Brazilin and the extract from Caesalpinia sappan L. protect oxidative injury through the expression of heme oxygenase‐1
Author(s) -
Choi ByungMin,
Lee JuA.,
Gao Shang Shang,
Eun Sang Yong,
Kim YoungSup,
Ryu ShiYong,
Choi YeonHee,
Park Raekil,
Kwon Dae Young,
Kim BokRyang
Publication year - 2007
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520300302
Subject(s) - heme , heme oxygenase , chemistry , programmed cell death , pharmacology , antioxidant , biochemistry , apoptosis , enzyme , medicine
In this study, we examined the protective effects of Caesalpinia sappan L. and its major component, brazilin, against tert‐butylhydroperoxide (t‐BHP)‐induced cell death in House Ear Institute‐Organ of Corti 1 (HEI‐OC1) cells. We found that the extract of C. sappan L. and brazilin induced antioxidant response element (ARE)‐luciferase activity and heme oxygenase‐1 (HO‐1) expression in a concentration‐dependent manner. The inductive effect of brazilin was more potent than the extract of C. sappan L. and the expression of HO‐1 reached a peak at 12 h after brazilin treatment. The extract and brazilin protected the cells against t‐BHP‐induced cell death. Their protective effects were abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results demonstrate that the extract of C. sappan L. and brazilin induce the expression of HO‐1 and the enzyme diminishes t‐BHP‐induced cell death in HEI‐OC1 cells.