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Macelignan protects HepG2 cells against tert ‐butylhydroperoxide‐induced oxidative damage
Author(s) -
Sohn Jong Hee,
Han Kyu Lee,
Choo Jeong Han,
Hwang JaeKwan
Publication year - 2007
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520290101
Subject(s) - dichlorofluorescein , comet assay , lipid peroxidation , chemistry , reactive oxygen species , viability assay , dna damage , malondialdehyde , lactate dehydrogenase , cytotoxicity , biochemistry , intracellular , microbiology and biotechnology , oxidative stress , mtt assay , cell damage , catalase , neutral red , cell growth , cell , enzyme , biology , dna , in vitro
In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert ‐butylhydroperoxide ( t ‐BHP)‐induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t ‐BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose‐dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t ‐BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates