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High Resolution 1 H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry
Author(s) -
Grootveld Martin,
Silwood Christopher J.L.,
Lynch Edward
Publication year - 2006
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520270102
Subject(s) - chemistry , formate , biomolecule , saliva , nuclear magnetic resonance spectroscopy , macromolecule , ozone , biochemistry , organic chemistry , catalysis
High resolution proton ( 1 H ) nuclear magnetic resonance (NMR) spectroscopy was employed to simultaneously evaluate the oxidising actions of ozone (O 3 ) towards a wide range of salivary biomolecules in view of its applications in dental practices, which may serve as a viable and convenient means for the treatment of dental caries. Treatment of supernatants derived from unstimulated human saliva specimens (n=12) with O 3 (4.48 mmol) revealed that this reactive oxygen species gave rise to the oxidative consumption of pyruvate (generating acetate and CO 2 as products), lactate (to pyruvate and sequentially acetate and CO 2 ), carbohydrates in general (a process generating formate), methionine (giving rise to its corresponding sulphoxide), and urate (to allantoin). Further, minor O 3 ‐induced modifications included the oxidation of trimethylamine and 3‐D‐hydroxybutyrate, the fragmentation of salivary glycosaminoglycans to NMR‐detectable saccharide fragments, and the conversion of polyunsaturated fatty acids to their ozonides. Moreover, evidence for the ability of O 3 to induce the release of selected low‐molecular‐mass salivary biomolecules from macromolecular binding‐sites was also obtained. Since many of the oxidation products detectable in O 3 ‐treated samples are identical to those arising from the attack of •OH radical on biofluid components, it appears that at least some of the modifications observed here are attributable to the latter oxidant (derived from O 3 •−generated from the single electron reduction of O 3 ).