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Activation of redox‐systems of monocytes by hydrogen peroxide
Author(s) -
Krjukov Alexei A.,
Semenkova Gali.,
Cherenkevich Sergei N.,
Gerein Valentin
Publication year - 2006
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520260406
Subject(s) - hydrogen peroxide , chemiluminescence , chemistry , superoxide , reactive oxygen species , stimulation , adhesion , monocyte , redox , biophysics , luminol , biochemistry , enzyme , immunology , endocrinology , inorganic chemistry , biology , chromatography , organic chemistry
In this work the influence of H 2 O 2 on the ability of human blood monocytes to generate ROS upon stimulation of cells by adhesion to glass surface and fMLP was studied using the luminol‐dependent chemiluminescence (LDCL) method. Pretreatment of cells with H 2 O 2 increased the adhesiveness of monocytes and ROS generation. Superoxide generation by cells in response to fMLP depended on the duration of pretreatment and the concentration of H 2 O 2 . The stimulatory effect on fMLP‐induced LDCL of cells further depended on the Ca 2+ concentration in the medium and on the activities of phospholipase A 2 , cyclooxygenases, and Mek1/2.