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S‐allyl cysteine attenuated CCl 4 ‐induced oxidative stress and pulmonary fibrosis in rats
Author(s) -
Mizuguchi Shinjiro,
Takemura Shigekazu,
Minamiyama Yukiko,
Kodai Shintaro,
Tsukioka Takuma,
Inoue Kiyotoshi,
Okada Shigeru,
Suehiro Shigefumi
Publication year - 2006
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520260108
Subject(s) - ccl4 , carbon tetrachloride , oxidative stress , chemistry , fibrosis , inflammation , pulmonary fibrosis , lung , pharmacology , nitric oxide , immunology , medicine , pathology , biochemistry , organic chemistry
This study examined effects of S‐allyl cysteine (SAC) on carbon tetrachloride (CCl 4 )‐induced interstitial pulmonary fibrosis in Wistar rats. CCl 4 (0.5 ml/kg) was intraperitoneally injected into rats twice a week for 8 weeks, and SAC (50, 100, or 200 mg/kg), N‐acetyl cysteine (NAC, 200 or 600 mg/kg), or L‐cysteine (CYS, 600 mg/kg) were orally administrated to rats everyday for 8 weeks. SAC significantly reduced the increases of transforming growth factor beta, lipid peroxides, AST, and ALT in plasma, induced by CCl 4 . Although CCl 4 is mainly metabolized by hepatic cytochrome P450, CCl 4 induced systemic inflammation and some organ fibrosis. SAC dose‐dependently and significantly attenuated CCl 4 ‐induced systemic inflammation and fibrosis of lung. SAC also inhibited the decrease of thiol levels, the increase of inducible nitric oxide synthase expression, the infiltration of leukocytes, and the generation of reactive oxygen species in lungs. Although NAC and CYS attenuated CCl 4 ‐induced pulmonary inflammation and fibrosis, the order of preventive potency was SAC > NAC > CYS according to their applied doses. These results indicate that SAC is more effective than other cysteine compounds in reducing CCl 4 ‐induced lung injury, and might be useful in prevention of interstitial pulmonary fibrosis.