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Estimation of plasma and saliva levels of coenzyme Q 10 and influence of oral supplementation
Author(s) -
Sekine K.,
Ota N.,
Nishii M.,
Uetake T.,
Shimadzu M.
Publication year - 2005
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520250125
Subject(s) - saliva , coenzyme q10 , chemistry , high performance liquid chromatography , human plasma , endocrinology , parotid gland , medicine , chromatography , biochemistry , dentistry
Coenzyme Q10 (CoQ 10 ) levels in human saliva were measured by HPLC with a highly sensitive electrochemical detector (ECD) and a special concentration column. This HPLC system showed satisfactory analytical results within the standard range of 0.78–50 ng/ml. We also found a significant correlation between CoQ 10 levels in plasma and in saliva from parotid glands, while this correlation was lacking between plasma CoQ 10 and CoQ 10 in whole saliva. Unlike in plasma, there are some fluctuations of saliva CoQ 10 levels throughout the day. A good correlation was obtained by colleting parotid gland saliva at times between meals. The mean saliva CoQ 10 level for 55 healthy volunteers was 17.0 ng/ml (S.D. 6.8 ng/ml); approximately one fiftieth of that in plasma. Regarding the influence of oral supplementation, CoQ 10 was analyzed in plasma and parotid gland saliva from 20 healthy volunteers supplemented daily with 100 mg of CoQ 10 for the first week and 200 mg for the second. The plasma CoQ 10 levels of all volunteers increased to different extents in accordance with the CoQ 10 daily intake and the corresponding change in saliva showed almost the same trend.