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CoQ 10 supplementation elevates the epidermal CoQ 10 level in adult hairless mice
Author(s) -
Ashida Yutaka,
Yamanishi Haruyo,
Terada Takatomo,
Oota Norihito,
Sekine Kyoichi,
Watabe Kazuo
Publication year - 2005
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520250120
Subject(s) - hairless , chemistry , biochemistry
We have already shown that prolonged supplementation of CoQ 10 in humans reduces the wrinkle area rate and wrinkle volume per unit area in the corner of the eye. CoQ 10 supplementation is known to increase the CoQ 10 level in serum and in many organs; however, the level of CoQ 10 in skin has not yet been fully investigated yet. We examined whether CoQ 10 intake elevates the CoQ 10 and CoQ 9 levels in epidermis, dermis, serum and other organs (kidney, heart, brain, muscle and crystalline lens) in 43‐week‐old hairless male mice. We also established a method using a high performance liquid chromatograph equipped with an electrochemical detector (HPLC‐ECD) to simultaneously quantify CoQ 9 and CoQ 10 in the tissues. CoQ 10 (0, 1, 100 mg/kg p.o.) was administered daily for 2 weeks. CoQ 10 supplementation of 100 mg/kg increased the serum and epidermal CoQ 10 levels significantly, but did not increase the CoQ 10 levels in either dermis or other organs. In conclusion, we showed that CoQ 10 intake elevates the epidermal CoQ 10 level, which may be a prerequisite to the reduction of wrinkles and other benefits related to the potent antioxidant and energizing effects of CoQ 10 in skin.