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Dihydroxynonene mercapturic acid, a urinary metabolite of 4‐hydroxynonenal, as a biomarker of lipid peroxidation
Author(s) -
Peiro GÉRaldine,
Alary Jacques,
Cravedi JeanPierre,
Rathahao Estelle,
Steghens JeanPaul,
Guéraud FranÇOise
Publication year - 2005
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520240110
Subject(s) - lipid peroxidation , 4 hydroxynonenal , metabolite , chemistry , malondialdehyde , mercapturic acid , urinary system , oxidative stress , urine , biomarker , chromatography , medicine , biochemistry , enzyme , cysteine
The objective of our study was to compare the information obtained through the use of three different urinary biomarkers of lipoperoxidation during the time course of a bromotrichloromethane (BrCCl 3 ) induced oxidative stress in rats. These biomarkers were malondialdehyde (MDA) measured by LC/MS after derivatization, the isoprostane 8‐iso‐PGF 2α measured by enzyme immunoassay and 1,4‐dihydroxynonene mercapturic acid (DHN‐MA), the major 4‐hydroxynonenal urinary metabolite [1], measured by LC‐MS. Male Wistar rats received a single dose of 100 μL/kg BrCCl3 per os and lipid peroxidation was estimated every day for a 4‐day‐period after treatment. MDA, 8‐iso‐PGF 2α and DHN‐MA significantly increased in response to BrCCl 3 treatment for this period of time, and DHN‐MA showed the main increase during the 24–48 h period after treatment.