Dihydroxynonene mercapturic acid, a urinary metabolite of 4‐hydroxynonenal, as a biomarker of lipid peroxidation | Zendy

Peiro GÉRaldine | Zendy; Alary Jacques | Zendy; Cravedi JeanPierre | Zendy; Rathahao Estelle | Zendy; Steghens JeanPaul | Zendy; Guéraud FranÇOise | Zendy

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Dihydroxynonene mercapturic acid, a urinary metabolite of 4‐hydroxynonenal, as a biomarker of lipid peroxidation

Author(s) -

Peiro GÉRaldine,

Alary Jacques,

Cravedi JeanPierre,

Rathahao Estelle,

Steghens JeanPaul,

Guéraud FranÇOise

Publication year - 2005

Publication title -

biofactors

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.204

H-Index - 94

eISSN - 1872-8081

pISSN - 0951-6433

DOI - 10.1002/biof.5520240110

Subject(s) - lipid peroxidation , 4 hydroxynonenal , metabolite , chemistry , malondialdehyde , mercapturic acid , urinary system , oxidative stress , urine , biomarker , chromatography , medicine , biochemistry , enzyme , cysteine

The objective of our study was to compare the information obtained through the use of three different urinary biomarkers of lipoperoxidation during the time course of a bromotrichloromethane (BrCCl 3 ) induced oxidative stress in rats. These biomarkers were malondialdehyde (MDA) measured by LC/MS after derivatization, the isoprostane 8‐iso‐PGF 2α measured by enzyme immunoassay and 1,4‐dihydroxynonene mercapturic acid (DHN‐MA), the major 4‐hydroxynonenal urinary metabolite [1], measured by LC‐MS. Male Wistar rats received a single dose of 100 μL/kg BrCCl3 per os and lipid peroxidation was estimated every day for a 4‐day‐period after treatment. MDA, 8‐iso‐PGF 2α and DHN‐MA significantly increased in response to BrCCl 3 treatment for this period of time, and DHN‐MA showed the main increase during the 24–48 h period after treatment.

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