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A novel test for identifying genes involved in aldehyde detoxification in the yeast. Increased sensitivity of superoxide‐deficient yeast to aldehydes and their metabolic precursors
Author(s) -
Biliński Tomasz,
Kwolek Magdalena,
Sas Ewelina,
Krynicka Marta,
Koziol Sabina,
OwsiakTeleon Alina,
Krzepilko Anna,
Bartosz Grzegorz
Publication year - 2005
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520240107
Subject(s) - yeast , detoxification (alternative medicine) , gene , biochemistry , aldehyde , chemistry , saccharomyces cerevisiae , superoxide , enzyme , medicine , catalysis , alternative medicine , pathology
A novel test for the identification of genes involved in aldehyde metabolism is proposed, based on detection of altered sensitivity of the yeast to corresponding alcohols, metabolic precursors of the aldehydes. This attitude enabled to an unexpected detection increased sensitivity of mutants devoid of CuZn‐superoxide dismutase (CuZnSOD) to allyl alcohol (precursor of acrolein) and nonenol. We interpret this finding as due to inactivation of some important element of aldehyde detoxification by increased flux of superoxide in ΔCuZnSOD mutants.