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Total hydroxyoctadecadienoic acid as a marker for lipid peroxidation in vivo
Author(s) -
Yoshida Yasukazu,
Hayakawa Mieko,
Niki Etsuo
Publication year - 2005
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520240102
Subject(s) - chemistry , saponification , sodium borohydride , in vivo , radical , lipid peroxidation , linoleic acid , biochemistry , chromatography , antioxidant , fatty acid , microbiology and biotechnology , biology , catalysis
An improved method for the measurement of lipid peroxidation in vivo has been recently developed, where total hydroxyoctadecadienoic acid (HODE) and 7‐hydroxycholesterol (FCOH) were determined by GC/MS analysis from physiological samples after reduction with sodium borohydride and saponification by potassium hydroxide. In this method, both free and ester forms of hydroperoxides and ketones as well as hydroxides of linoleic acid and cholesterol are measured as HODE and FCOH, respectively. The ratio of stereo‐isomer, (Z, E)‐HODE/(E, E)‐HODE, could be also measured. In the present study, in order to examine the effect of continuous, slow flux of free radicals in vivo, a water‐soluble radical generator was administered to rats and mice and the amounts of HODE and 8‐isoprostane in plasma and liver were measured. It was found that the administration of free radical‐generating azo compound increased the level of HODE and decreased the (Z, E)‐HODE/(E, E)‐HODE ratio in both plasma and liver. The level of HODE was much higher than 8‐isoprostane.