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Simple and rapid determination of 1‐deoxynojirimycin in mulberry leaves
Author(s) -
Kimura Toshiyuki,
Nakagawa Kiyotaka,
Saito Yuko,
Yamagishi Kenji,
Suzuki Masahiro,
Yamaki Kohji,
Shinmoto Hiroshi,
Miyazawa Teruo
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520220167
Subject(s) - chromatography detector , chromatography , chemistry , detection limit , mass spectrometry , amide , column chromatography , high performance liquid chromatography , biochemistry
A simple and rapid method for determining 1‐deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves ( Morus alba and Morus bombysis ), by high performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from extract of mulberry leaves on TSK gel Amide‐80 column, which is a representative column for hydrophilic interaction chromatography. During post column detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.