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Quantitative determination of urinary 8‐hydroxy‐2′‐deoxyguanosine level in healthy Japanese volunteers
Author(s) -
Miwa Misao,
Matsumaru Hiroyuki,
Akimoto Yukari,
Naito Shigehiro,
Ochi Hirotomo
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520220150
Subject(s) - urine , morning , urinary system , 8 hydroxy 2' deoxyguanosine , creatinine , oxidative stress , medicine , deoxyguanosine , urine sample , physiology , dna oxidation , oxidative damage
8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), as a measure of oxidative stress, was measured in healthy Japanese volunteers using an ELISA (New 8‐OHdG Check®, JICA). Analysis of daytime spot urine of 83 healthy male subjects and smoking habit, exercise and age revealed significant correlation only between the urinary level of 8‐OHdG and age. As the inter‐individual variation of 8‐OHdG of the daytime spot urine was relatively high, we next determined inter‐and intra‐individual variation of 5 healthy volunteers. The levels of 8‐OHdG/creatinine in morning spot urine significantly correlated with 8‐OHdG levels in 24‐h pool urine. Thus, a morning spot urine sample can be used for the measurement of 8‐OHdG instead of inconvenient 24‐h sampling.