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High‐throughput profiling of dietary polyphenols and their metabolites by HPLC‐ESI‐MS‐MS in human urine
Author(s) -
Ito Hideyuki,
Gonthiera MariePaule,
Manach Claudine,
Morand Christine,
Mennen Louise,
Rémésy Christian,
Scalbert Augustin
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520220148
Subject(s) - polyphenol , chromatography , urine , chemistry , electrospray ionization , high performance liquid chromatography , mass spectrometry , electrospray , antioxidant , biochemistry
Polyphenols in biological fluids are generally estimated by HPLC with UV, electrochemical or fluorimetric detection. We describe herein a method specially developed to estimate biomarkers of polyphenol consumption in human urine. We simultaneously quantified 15 polyphenols and their related compounds in human urine using an HPLC coupled with electrospray ionization mass‐mass (HPLC‐ESI‐MS‐MS) method with an analytical run time of 6 min. The method has been validated with respect to linearity, precision, and accuracy in intra‐ and inter‐day assays. It has been applied to human urine samples collected from volunteers after consumption of different polyphenol‐rich beverages in controlled conditions.