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Effect of quercetin conjugates on vascular permeability and expression of adhesion molecules
Author(s) -
Mochizuki Mika,
Kajiya Katsuko,
Terao Junji,
Kaji Kazuhiko,
Kumazawa Shigenori,
Nakayama Tsutomu,
Shimoi Kayoko
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520220142
Subject(s) - quercetin , chemistry , glucuronide , conjugate , in vivo , biochemistry , liposome , antioxidant , aglycone , in vitro , intracellular , pharmacology , biology , stereochemistry , metabolite , glycoside , mathematical analysis , mathematics , microbiology and biotechnology
Quercetin and its glucosides exist in plant foods and are recovered in human plasma as glucuronide and sulfate conjugates. Quercetin and its conjugates show antioxidant activity in model experiments. It remains obscure, however, whether these conjugates retain these biological functions in vivo. We investigated the interaction of quercetin conjugates with lipid bilayers using liposome systems. Less quercetin conjugate was incorporated into liposomes than quercetin aglycone. We also studied the vascular permeability of quercetin‐3‐glucuronide using cell culture inserts. Incubation of human aortic endothelial cells (HAECs) with IL‐1α resulted in increased permeability of quercetin‐3‐glucuronide. Furthermore, quercetin‐3‐glucuronide showed no suppressive effect on TNF‐α‐induced cell expression of intercellular adhesion molecule‐1 (ICAM‐1) on HAECs. These observations suggest that circulating conjugates of quercetin pass through the endothelium to reach vascular smooth muscle cells and exert their biological effects in the blood vessels during inflammation followed by deconjugation of the conjugates.