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Cytoplasmic fraction of Lactococcus lactis ssp. lactis induces apoptosis in SNU‐1 stomach adenocarcinoma cells
Author(s) -
Kim Seo Young,
Lee Ki Won,
Kim Ji Yeon,
Lee Hyong Joo
Publication year - 2004
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520220123
Subject(s) - lactococcus lactis , apoptosis , cytoplasm , microbiology and biotechnology , adenocarcinoma , chemistry , lactococcus , stomach , biology , bacteria , biochemistry , lactic acid , cancer , genetics
Lactic acid bacteria are known to have antitumor activity, but the underlying mechanisms remain unclear. Recently we showed that a cytoplasmic fraction ‐ but not peptidoglycan ‐ of Lactococcus lactis ssp. lactis (L.lac CF) had strong antiproliferative activity on SNU‐1 human stomach adenocarcinoma cells. The present study investigated whether the antiproliferative activity of L.lac CF on SNU‐1 is linked to the induction of apoptosis. Treatment of L.lac CF inhibited the proliferation of SNU‐1 cells in a dose‐ and time‐dependent manner. Furthermore, treatment of the cells with 50 μ/ml and 100 μ/ml L.lac CF resulted in DNA fragmentation and chromatin condensation, respectively. The results indicate that the inhibitory effect of L.lac CF on SNU‐1 cell growth is mainly attributable to the induction of apoptosis.