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Characteristic rat tissue accumulation of nobiletin, a chemopreventive polymethoxyflavonoid, in comparison with luteolin
Author(s) -
Murakami Akira,
Koshimizu Koichi,
Ohigashi Hajime,
Kuwahara Shigeru,
Kuki Wataru,
Takahashi Yasuo,
Hosotani Keisuke,
Kawahara Satsuki,
Matsuoka Yumi
Publication year - 2002
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520160303
Subject(s) - luteolin , nobiletin , chemistry , flavones , flavonoid , kidney , quercetin , urine , pharmacology , endocrinology , biochemistry , biology , chromatography , antioxidant
Nobiletin (NOB), a polymethoxyflavonoid, is an effective anti‐inflammatory and chemopreventive phytochemical found in citrus fruits. We compared the absorption and metabolism characteristics of NOB with those of luteolin (LT) in male SD rats. Each flavonoid (67.1 μmol/kg of body weight) was given separately by gastric intubation, and then concentrations were measured at 1, 4, and 24 hours after administration. In the digestive organs, NOB showed a notable tendency for localizing into the mucous membrane and muscularis from 1 to 4 hours, in contrast to LT, though both NOB and LT were completely excreted within 24 hours. Further, significant amounts of NOB were detected in the whole liver and kidney specimens, whereas LT accumulation was slight. Although serum concentrations of NOB from 1 to 4 hours were comparable to those of LT, urinary concentrations of LT were significantly higher from 4 to 24 hours. Following glucuronidase/sulfatase treatments of urinary materials, we detected 3 types of mono‐demethylated NOB, including 3′‐demethyl‐NOB, and two di‐demethylated types, as well as 3′‐demethyl‐NOB alone in serum samples using liquid chromatography‐mass spectral analysis. Our results suggest that the metabolic properties of polymethoxyflavonoids are distinct from those of other general flavonoids, because of their wide distribution and accumulation in tissue.