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Modulation of steroidogenesis by selenium in a novel adrenal cell line developed using targeted tumorigenesis
Author(s) -
Chanoine JeanPierre,
Compag Nathalie A.,
Wong Alfred C. K.,
Mellon Synthia H.
Publication year - 2001
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520140129
Subject(s) - selenium , endocrinology , medicine , glutathione peroxidase , aldosterone , corticosterone , chemistry , steroid hormone , hormone , mineralocorticoid , selenium deficiency , steroid , selenoprotein , biology , oxidative stress , superoxide dismutase , organic chemistry
Abstract Glutathione peroxidase (GPx‐1) is a selenoenzyme that metabolizes H 2 O 2 , a source of potentially toxic free radicals. Steroidogenesis is markedly inhibited by H 2 O 2 in vitro. Objective: to study the effects of selenium deficiency on GPx activity and adrenal steroidogenesis in a novel adrenal cell line developed using targeted tumorigenesis. Methods: AN4Rppc7 cells were grown for 7 days in serum‐free medium. 8‐Br‐cAMP‐stimulated concentrations of steroid hormones were measured by RIA. StAR (Steroid Acute Reactive Protein) mRNA was measured by Northern blot. Results: selenium deficiency caused a 99% There was a 51%, progesterone, corticosterone and aldosterone production, respectivelyp<0.05 by ANOVA). StAR mRNA was not affected by selenium. Conclusions: selenium deficiency causes a marked decrease in GPx activity. Decreased steroid hormone production occurs for selenium concentrations equal or lower than 5 nM. The absence of changes in StAR mRNA content suggests that selenium deficiency does not affect cholesterol access to the mitochondria.