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Stability of gastrointestinal glutathione peroxidase mRNA in selenium deficiency depends on its 3′UTR
Author(s) -
Wingler Kirstin,
Müller Cordula,
BrigeliusFlohé Regina
Publication year - 2001
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520140107
Subject(s) - glutathione peroxidase , messenger rna , untranslated region , gpx1 , three prime untranslated region , microbiology and biotechnology , selenium , chemistry , gpx3 , ebselen , peroxidase , selenoprotein , glutathione , biology , biochemistry , enzyme , gene , organic chemistry
Selenoproteins decrease upon selenium‐deprivation according to their hierarchical ranking. Whereas classical glutathione peroxidase (cGPx) responds to decreased selenium supply with a complete loss of protein and a marked reduction of mRNA levels, gastrointestinal glutathione peroxidase (GI‐GPx) remains detectable and its mRNA is stable. The impact of the 3′UTR on cGPx and GI‐GPx mRNA stability was studied in stably transfected HepG2 cells with combinations of mutually exchanged coding regions and 3′UTRs of human cGPx and GI‐GPx. Stability of chimeric mRNAs was measured by competitive RT‐PCR. We found that GI‐GPx 3′UTR is sufficient to stabilize its own mRNA but not that of cGPx.