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Antitumor protein (AP) from a mushroom induced apoptosis to transformed human keratinocyte by controlling the status of pRb, c‐MYC, cyclin E‐cdk2, and p21 WAF1 in the G1/S transition
Author(s) -
Kawamura Yukio,
Manabe Mariko,
Kitta Kazumi
Publication year - 2000
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520120125
Subject(s) - apoptosis , cyclin dependent kinase 2 , cyclin , cyclin e , cancer research , cyclin dependent kinase , keratinocyte , microbiology and biotechnology , cyclin a , chemistry , cell cycle , biology , biochemistry , in vitro
Antitumor protein (AP) from a mushroom, induced the morphological changes typical to apoptosis such as nuclear condensation, aneuploidity, and DNA fragmentation at concentrations as low as 5–20 ng/ml to cancer cells. Molecular alterations related to cell cycle. Molecular alterations related to cell cycle, especially G1/S transition were investigated with a human keratinocyte transformed with oncoproteins, E6 and E7 of human pappiloma virus(HPV)‐16. AP didn't alter significantly and oncosuppressor p53 level, but induced hyperphosphorylation of pRb. Time‐dependent change of G1 cyclins, cdk2 and cdk4 after addition of AP showed that expression level of cdk inhibitors, INK4 family, and p27 KIP1 did not altered, while that of p21 WAF1 was downregulated.