Premium
Effects of L‐lysine deficient diet on the hypothalamic interstitial norepinephrine and diet‐induced thermogenesis in rats in vivo
Author(s) -
Smriga M.,
Murakami H.,
Mori M.,
Torii K.
Publication year - 2000
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520120121
Subject(s) - microdialysis , medicine , endocrinology , thermogenesis , in vivo , hypothalamus , lysine , norepinephrine , chemistry , amino acid , biology , central nervous system , biochemistry , obesity , dopamine , microbiology and biotechnology
Abstract Rats readily recognize an amino acid deficient diet, presumably via central mechanisms that involve hypothalamic circuits. Presently, effects of the essential amino acid L‐lysine deficiency on the ventromedial (VMH) and lateral (LH) hypothalamus norepinephrine (NE) release were evaluated in free moving rats. Microdialysis measurement was undertaken once in 48 h (12:00 noon–14:00) in rats that had free access to food and drink. Significant decline in the food intake and VMH NE release were found in rats fed L‐lysine diet. No changes were identified in LH NE release. Additionally, no significant differences in diet‐induced spatial thermogenesis between normal and L‐lysine deficient non‐stressed rats were found in vivo. The results suggested that the VMH NE release was specifically involved in the integration of signals about amino acid deficiency. However, the decrease in VMH NE was not translated into changes of thermogenic responses to diet.