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Electron microscopic tomography of rat‐liver mitochondria and their interactions with the endoplasmic reticulum
Author(s) -
Mannella Carmen A.,
Buttle Karolyn,
Rath Bimal K.,
Marko M.
Publication year - 1998
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520080309
Subject(s) - endoplasmic reticulum , organelle , electron tomography , mitochondrion , microbiology and biotechnology , chemistry , biophysics , membrane , tomographic reconstruction , tomography , electron microscope , biochemistry , biology , physics , scanning transmission electron microscopy , optics
Electron microscopic tomography can provide a complete three‐dimensional range of information about subcellular structures. Applied to mitochondria, it has shown that the conventional textbook model of this organelle is incorrect. The infoldings of the inner membrane (called cristae) are connected by narrow tubular regions to each other and to the outside, suggesting that internal diffusion of ions, metabolites and proteins may be restricted. Tomographic reconstruction of mitochondria in situ indicates these organelles may occur in clusters with stacks of endoplasmic reticulum, forming extended structures that may be involved in [Ca ++ ] regulation.