Premium
Cytotoxicity of paraquat in freshly isolated rat hepatocytes: effects of L‐carnitine
Author(s) -
GarcíaRubio L.,
GarcíaAbad A. M.,
Soler F.,
Míguez M. P.
Publication year - 1998
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520080111
Subject(s) - cytotoxicity , paraquat , pharmacology , chemistry , carnitine , biochemistry , biology , in vitro
The effects of L‐carnitine, a mitochondrial carrier of fatty acids, on paraquat (PQ) cytotoxicity in freshly isolated rat hepatocytes were studied. Addition of PQ (10 mM) to hepatocytes resulted in a time‐dependent depletion of intracellular glutathione (GSH) accompanied by an increase in accumulation of malondialdehyde (MDA) in the incubation medium which preceeded to a loss of cell viability. Pretreatment of hepatocytes with L‐carnitine (50 mM) alone did not affect cell viability or intracellular levels of GSH, or accumulation of MDA in the medium during the incubation period; however, pretreatment with L‐carnitine 30 min prior to PQ addition did promote the depletion of intracellular GSH and MDA accumulation induced by PQ, and ultimately enhanced the cytotoxicity of PQ.