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Biogenic 4‐hydroxy‐2‐nonenal activates transcription factor AP‐1 but not NF‐κB in cells of the macrophage lineage
Author(s) -
Camandola Simonetta,
Scavazza Antonella,
Leonarduzzi Gabriella,
Biasi Fiorella,
Chiarpotto Elena,
Azzi Angelo,
Poli Giuseppe
Publication year - 1997
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.5520060211
Subject(s) - activator (genetics) , transcription factor , chemistry , nfkb1 , nf κb , cell culture , microbiology and biotechnology , lipid peroxidation , transcription (linguistics) , in vitro , biochemistry , gene , apoptosis , biology , oxidative stress , genetics , linguistics , philosophy
A large spectrum of pro‐oxidant agents, including molecules with lipoperoxidative effect, can modulate gene expression through modification of the DNA binding activity of the transcription factors activator protein 1 (AP‐1) and nuclear factor kappa B (NF‐κB). In this study the effect on these redox‐sensitive factors by 4‐hydroxy‐2‐nonenal (HNE), a major aldehydic product of lipid peroxidation, was examined in two cell lines of the macrophage type. Incubation in the presence of μM concentrations of the aldehyde led to a rapid increase of AP‐1 binding with a transient maximum 30 min from HNE addition to the culture medium in both cell lines. On the contrary, HNE did not stimulate nuclear translocation of NF‐κB. The diverging effect of HNE on the two transcription factors is likely related to the demonstrated differential activation pathway of AP‐1 and NF‐κB in macrophages. The HNE‐induced activation of AP‐1 suggests the aldehyde's involvement in the regulatory mechanisms of cell proliferation and differentiation.