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Antioxidant and reducing lipid accumulation effects of rutin in Caenorhabditis elegans
Author(s) -
Qin Xianjin,
Wang Wenqian,
Chu Weihua
Publication year - 2021
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1755
Subject(s) - rutin , caenorhabditis elegans , lipid metabolism , antioxidant , food science , chemistry , fatty acid , triglyceride , biochemistry , biology , cholesterol , gene
In this study, the effect of rutin on fatty acid metabolism and antioxidant activity were evaluated. We found that the antioxidant capacity of rutin‐treated Caenorhabditis elegans was enhanced but the triglyceride content was significantly reduced. The reduction of fat accumulation by rutin was also confirmed by Oil Red O staining. RNA‐seq analysis indicated that rutin significantly regulated the expression of seven genes related to lipid metabolism in C. elegans . Among the seven genes, acox‐1.3 , stdh‐3 , and fat‐7 were associated with fatty acid metabolism. Rutin significantly reduced fat accumulation in both fat‐6 and fat‐7 mutant strains but did not affect the fat storage of fat‐6 / fat‐7 double mutant, which indicated that the impact of rutin on fat storage depended on fat‐6 and fat‐7 . These findings indicated that rutin reduced fat storage depending on the regulation of lipid metabolism‐related genes expression and thereby regulating the biosynthesis of the corresponding unsaturated fatty acid.