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Interleukin‐9 deficiency affects lipopolysaccharide‐induced macrophage‐related oxidative stress and myocardial cell apoptosis via the Nrf2 pathway both in vivo and in vitro
Author(s) -
Liang Zhishan,
Pan Fuze,
Yang Zicong,
Wang Mengjie,
Hu Changxing,
Shi Lei,
Ji Qingwei,
Liu Ling
Publication year - 2021
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1754
Subject(s) - apoptosis , oxidative stress , lipopolysaccharide , chemistry , endocrinology , p38 mitogen activated protein kinases , in vivo , knockout mouse , interleukin , medicine , inflammation , cytokine , immunology , biology , signal transduction , biochemistry , receptor , mapk/erk pathway , microbiology and biotechnology
Previous studies showed that interleukin‐9 (IL‐9) is involved in cardiovascular diseases, including hypertension and cardiac fibrosis. This study aimed to investigate the role of IL‐9 in lipopolysaccharide (LPS)‐induced myocardial cell (MC) apoptosis. Mice were treated with LPS, and IL‐9 expression was measured and the results showed that compared with WT mice, LPS‐treated mice exhibited increased cardiac Mø‐derived IL‐9. Additionally, the effects of IL‐9 deficiency (IL‐9−/−) on macrophage (Mø)‐related oxidative stress and MC apoptosis were evaluated, the results showed that IL‐9 knockout significantly exacerbated cardiac dysfunction, inhibited Nrf2 nuclear transfer, promoted an imbalance in M1 and M2 Møs, and exacerbated oxidative stress and MC apoptosis in LPS‐treated mice. Treatment with ML385, a specific nuclear factor erythroid‐2 related factor 2 (Nrf2) pathway inhibitor significantly alleviated the above effects in LPS‐treated IL‐9−/− mice. Bone marrow‐derived Møs from wild‐type (WT) mice and IL‐9−/− mice were treated with LPS, and the differentiation and oxidative stress levels of Møs were measured. The effect of Mø differentiation on mouse MC apoptosis was also analyzed in vitro. The results showed that LPS‐induced M1 Mø/M2 Mø imbalance and Mø‐related oxidative stress were alleviated by IL‐9 knockout but were exacerbated by ML385 treatment. The protective effects of IL‐9 deficiency on the MC apoptosis mediated by LPS‐treated Møs were reversed by ML‐385. Our results suggest that deletion of IL‐9 decreased the nuclear translocation of Nrf2 in Møs, which further aggravated Mø‐related oxidative stress and MC apoptosis. IL‐9 may be a target for the prevention of LPS‐induced cardiac injury.