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Epidermal growth factor/epidermal growth factor receptor moves into the osteoblasts' cell nuclei where it is involved to regulate STAT5 's signaling
Author(s) -
Yin Bohao,
Fan Zhiyuan,
Jin Yingzhe,
Chen Hongchi,
Zhang Baokun,
Liu Jingwen,
Zhang Wei
Publication year - 2021
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1709
Subject(s) - microbiology and biotechnology , epidermal growth factor receptor , epidermal growth factor , chemistry , stat5 , growth factor , co receptor , signal transduction , growth factor receptor , receptor , biology , biochemistry
Epidermal growth factor (EGF) has vital biological impacts on the osteoblasts. However, the knowledge on the cellular properties of EGF/EGFR (epidermal growth factor receptor) on osteoblasts is scanty. As such, we explored the EGF/EGFR's cell behavior in the osteoblast (MC3T3‐E1 cell) using the indirect immunofluorescence assay, Western‐blot, and fluorescence resonance energy transfer. Our findings revealed that EGF could internalize into the cytoplasm under EGFR mediation. Besides, the co‐localization analysis demonstrated that caveolin played a critical role in EGFR's endocytosis. We also analyzed the cytoplasmic trafficking pathway of EGF/EGFR in MC3T3‐E1 cell. The colocalization analysis showed that EGFR entered into Rab5, Rab4, and Rab9‐positive endosomes. More importantly, we found that EGFR could move into the MC3T3‐E1 cells' nuclei. Based on this, we investigated the EGFR's nuclear‐localized functions, and the results suggested that nuclear‐localized EGFR has important biological functions. This work lays a foundation for further study on EGF/EGFR's biological functions on the osteoblasts.