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Maternal vitamin D deficiency increases the thromboxane/prostacyclin ratio through alterations in the one‐carbon cycle in Wistar rats
Author(s) -
Nandi Anindita A.,
Wadhwani Nisha S.,
Joshi Sadhana R.
Publication year - 2019
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1510
Subject(s) - medicine , endocrinology , prostacyclin , thromboxane , cyclooxygenase , thromboxane a2 , chemistry , vitamin d deficiency , placenta , prostaglandin , vitamin d and neurology , biology , enzyme , fetus , pregnancy , platelet , biochemistry , genetics
This study aims to test the hypothesis that vitamin D deficiency can influence long‐chain polyunsaturated fatty acid metabolism through alterations in the one‐carbon cycle. Wistar rats ( n = 8 per group) were given either a control (1,000 IU D3/kg diet) or a vitamin D deficient (VDD) (0 IU D3/kg diet) diet from pre‐pregnancy to delivery. On day 20 of gestation, pregnant female rats were delivered by C‐section to collect placenta and blood. VDD group demonstrated high serum parathyroid hormone, low serum phosphate, low plasma folate, higher plasma homocysteine, and higher plasma malondialdehyde levels ( P < 0.05 for all) as compared to control. Lower protein levels of placental cystathionine‐β‐synthase enzyme ( P < 0.05) were observed in the VDD group as compared to control. VDD group demonstrated higher placental mRNA levels of the enzymes phospholipase A 2 and cyclooxygenase‐2 ( P < 0.05 for both) as compared to control. Protein levels of the enzymes phospholipase A 2 and cyclooxygenase‐2 were lower ( P < 0.05 for both) in the VDD group as compared to the control group. The ratio of thromboxane B 2 and 6‐keto prostaglandin F 1α in serum was higher ( P < 0.05) in the VDD group as compared to control; although the serum levels of 6‐keto prostaglandin F 1α and thromboxane B 2 were similar in both the groups. Our findings suggest that increased oxidative stress due to maternal vitamin D deficiency results in the imbalance between the vasoconstrictor (thromboxane B 2 ) and vasodilator (6‐keto prostaglandin F 1α ) eicosanoids, which may lead to endothelial dysfunction and poor pregnancy outcome. © 2019 BioFactors, 45 (4):548–555, 2019