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Neuroprotection of mangiferin against oxidative damage via arousing Nrf2 signaling pathway in PC12 cells
Author(s) -
Peng Shoujiao,
Hou Yanan,
Yao Juan,
Fang Jianguo
Publication year - 2019
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1488
Subject(s) - neuroprotection , mangiferin , oxidative stress , chemistry , pharmacology , neurodegeneration , antioxidant , programmed cell death , microbiology and biotechnology , gene knockdown , biochemistry , biology , medicine , apoptosis , disease
Accumulating evidence demonstrates that oxidative stress is involved in the pathogenesis and progression of neurodegeneration. As NF‐E2‐related factor 2 (Nrf2) plays a crucial role in maintaining cellular redox homeostasis, small molecules with the ability in activation of Nrf2 pathway are promising neuroprotective agents. Mangiferin (Mg) is a xanthone glucoside extracted from mangoes and papayas, and has been reported to possess multiple pharmacological activities. In this study, we investigated neuroprotective effects of Mg in the neuron‐like rat pheochromocytoma cell line (PC12 cells). Mg scavenges different kinds of free radicals in vitro and attenuates hydrogen peroxide‐ or 6‐hydroxydopamine‐induced cell death. After treatment with Mg, a range of antioxidant genes governed by Nrf2 were upregulated, and the expressions and activities of these gene products were also elevated. Moreover, knockdown of Nrf2 antagonized the protective effect of Mg, indicating that Nrf2 is an essential factor in this cytoprotective process. In summary, our study demonstrates that Mg is a potent antioxidant that can provide neuroprotection against oxidative stress‐mediated damage of PC12 cells. © 2019 BioFactors, 45(3):381–392, 2019