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Anti‐inflammatory, antioxidant, and immunomodulatory effects of curcumin in ovalbumin‐sensitized rat
Author(s) -
Shakeri Farzaneh,
Boskabady Mohammad Hossein
Publication year - 2017
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1364
Subject(s) - ovalbumin , curcumin , eosinophil , dexamethasone , antioxidant , bronchoalveolar lavage , chemistry , immunoglobulin e , sensitization , pharmacology , oxidative stress , immunology , lymphocyte , medicine , endocrinology , asthma , immune system , biochemistry , antibody , lung
Background : Anti‐inflammatory and antioxidant properties of curcumin have been shown. In this study, anti‐inflammatory, antioxidant, and immunomodulatory effects of curcumin in sensitized rat were evaluated. Methods : Six groups of rats including control (C), ovalbumin‐sensitized (as a rat model of asthma, S), S groups treated with curcumin (Cu 0.15, 0.30, and 0.60 mg/mL), and 1.25 μg/mL dexamethasone (S + D) were studied. Curcumin and dexamethasone were given in animals' drinking water during sensitization period. Total and differential WBC count, PLA2, TP, IFN‐γ, IL‐4, IgE, oxidant, and antioxidant biomarker levels in bronchoalveolar lavage fluid (BALF) were examined. Results : Total WBC, neutrophil and eosinophil counts, levels of PLA2, TP, IgE, IL‐4, NO 2 , NO 3 , and MDA in BALF were increased but lymphocyte percentage, SOD, CAT, thiol, and IFN‐γ levels and IFN‐γ/IL‐4 ratio decreased in S animals compared to controls ( P < 0.001 for all cases). Treatment with all concentrations of curcumin significantly improved total WBC, PLA2, TP, IgE, IL‐4, IFN‐γ, IFN‐γ/IL‐4 ratio, SOD, thiol, NO 2 , and NO 3 compared to S group ( P < 0.01 to P < 0.001). Two higher concentrations of curcumin significantly decreased neutrophil and eosinophil counts and MDA level but increased IFN‐γ, CAT and lymphocyte values compared to S group ( P < 0.001 for all cases). Dexamethasone treatment also significantly improved most of the measured parameters ( P < 0.05 to P < 0.001) but it did not change IL‐4 and IFN‐γ levels and IFN‐γ/IL‐4 ratio. Conclusion : Anti‐inflammatory, antioxidant, and immunomodulatory effects of curcumin with more specific immunomodulatory effect on Th1/Th2 balance compared to dexamethasone in sensitized rats was shown. © 2017 BioFactors, 43(4):567–576, 2017